A Pasteur pipette dropper is a relatively simple diaphragm in the hands of biologists, scientists and other professionals who need to work with small amounts of liquids. The method is developed by a famous French microbiologist that was named Louis Pasteur then the term pipette because it's created in 1857. The dropper is essentially a very slender glass tube that neatly helps in transferring trace amounts of liquid from one place to another.
At the end, you have a large opening and can simply put your Pasteur dropper into each of them. The capacity of a Pasteur pipette dropper ranges from 50 µL to 1000 µL, but keep in mind that while they are convenient tools for relatively quick liquid transfer applications, these are not as accurate ly utilized as the micropipetters reliably used here for precise analytical work.
To use with a Pasteur pipette dropper REFER(work in progress ) Cleaning phase for the pasteur Pipettes;-Step 1 : -Conceptual sterilization process. That includes putting the dropper into a disinfectant solution followed by heating with a Bunsen burner. The dropper can then be used to transfer fluids (by getting tip into liquid container and sucking it up) In order to use, simply squeeze the bulb at top of dropper until liquid appears.
Creative Ideas for the Quantification of Minute Liquid Volumes using a Pasteur Pipette Dropper
Methods to measure very small liquid volumes using Pasteur pipette droppers may be adopted creatively. For the latter one there is an even more complicated procedure which we must fit the values into a standard curve of some compound. This procedure involves the dissolution of a predetermined quantity of the material in an identified volume. Then, a few dilutions are prepared with the pipette and each time is less concentrated in compound. This sets up a standard curve, with this as the x-axis coordinate and absorbance at 450nM on the y-Coordinate which researchers can then use to infer concentrations of an unknown compound.
Unusual creative approach - is an assessment of the density of liquids with the help Pasteur pipette dropper. The dropper is first weighed empty and then again filled with a specific volume of liquid so they can estimate the density. The difference in weight between the two readings is proportional to volume of the liquid.
Pasteur Glass Pipette Droppers are very useful for laboratory purpose. These are relatively cheap and easy to acquire, so many scientists and researchers use them. In addition, they are easy to use and more accessible for people in general who may not have the experience with other experimental methods. Also, since they can be used for different applications (titrations or extractions), it makes them capable of drawing up one amount and dispense another.
Advantages and disadvantages of reuse for Pasteur pipette droppers They must be replaced after each use, and reusing droppers could save you time as well as money. Nonetheless, using this policy may result in contamination of the experiments and reduced accuracy. This risk is exacerbated when working with sensitive samples such as DNA or RNA, where even low levels of contamination can lead to drastic changes in experimental results. On top of it potentially affecting the accuracy or results, there comes another edge if one re-uses droppers: breakage and damage.
As of now some kind of dropper/cap is available with the Pasteur pipette so that makes it easy to drop things, sometimes there are different types which serve many purposes. Cotton-plugged dropper which is a type of common benedict's reagent with cotton wool to stop contaminants[]. This is best to be used while dispensing and transfer of liquids that do not easily get contaminated.
One of these is the plastic dropper, which unlike glass made plastics. This disposable dropper is ideal for transferring and dispensing sensitive samples needing high sterility. Besides this, plastic droppers are light and easy to handle.
Pasteur pipette droppers are important tools for all scientists in the fields of bio, chemical and medical sciences. These scales provide a ready-to-go user-friendly solution suitable for everyday versatile use in the laboratory. The Pasteur pipette dropper goes a long way in measuring liquid volumes to making standard curves for compounds. Sterile technique must be preserved and cross contamination avoided with everything that the scientist touches when manipulating sensitive samples.
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