HOW TO STERILIZE PASTEUR PIPETTES FOR CELL CULTURE
Consequently they have to be cleaned really well before using Pasteur pipettes to prevent germs or contamination in the cell culture. Letsvečkanalni rezervoar za pipeto call this cleaning process sterilizing. Shengke has Pasteur pipettes already cleaned and ready to use, which is a convenience for scientists. But if you do them yourself, here are some useful tips to get it right:
Pipettes: Place the pipettes in a clean autoclave bag and sterilize. Then heat them at 121°C for 20 minutes. This will destroy any germsvečkanalna pipeta rezervoarja and ensure they’re safe to use.
Dry heat: If cleaning in the presence of water is not suitable, you can always place the pipettes in a dry oven. You must cook them at 160°Crezervoar reagenta za večkanalne pipete for two hours. This technique also works well for sterilization.
Chemical cleaning: Alternatively, you can immerse the pipettes in a special solution. 70% alcohol or 10% bleach for 30 minutes Once soaked, it’s necessary to rinse them with clean and sterile water to wash away any excess chemicals.
Contaminating, Aspirating, And Dispensing
In cell culture, scientists typically perform three main actions with Pasteur pipettes for working with liquids: transferring (moving a liquid from one container to another), aspirating (drawing a liquid into a Pasteur pipette), and dispensing (releasing the liquid from the Pasteur pipette). Let’s take a closer look at each of those actions.
