PCR well plate technology. How Many Of You Aware PyQt? This describes a trick by some scientists who study small pieces of DNA very smart behavior. DNA (Deoxyribo Nucleic Acid) is genetic material common to all living creatures. Scientists mix chemicals and DNA together in small tubes called PCR (polymerase chain reaction) tubes in the lab. These tubes are great, but most of the time enough samples to assay hundreds or even thousands of different DNA varieties simultaneously. But then they go to PCR well plates in those instances!
PCR well plates are small plastic dishes that have tiny holes in them, wells. Each individual well can hold only a small amount of sample or chemical This way, scientists can place a drop of only four various samples in each hole on the plate. After samples are inside the wells, scientists ask a special machine to over and again heat it up & cool down This is crucial because this step phosphorylates the DNA in each well and multiplies it many times over for further examination by scientists.
PCR well plates are cool, they save ton of time and materials. In one part of the well plate (donor) Labs can test multiple samples at once, testing using every DNA sample in an individual tube could take a lot longer. It means they can run their experiments faster, which is necessary because the wear and tear on them is very difficult to replace. Recall that it is eco friendly as there are lesser amount of plastic and chemicals used in this.
As a plate-based method it is also faster, and keeps everything clean. Just a number we put on the tube, and something only scientists need to keep track of sample location - even in that weird well. This is essentially to keep everyone organized and prevent any human errors that might occur were they to mix up the samples. Organized: In future, you can always compare the results. The more precisely a scientist can pinpoint where individual samples are located within any given data, the better they will be able to analyze it in service of their work.
After warming and cooling the samples in one of their well plates, the scientists now want to look at this DNA. Well plates are also a method by which we can test many samples at one time, effectively transferring our sample from one plate to another. To accomplish this, scientists will use a multi-channel pipette device. When pressurized, they can transfer extremely minute quantities of liquid from one point to another in a droplet free manner and with nanolitre scale accuracy. Working at the level of DNA, it is key to have this precision in place, they said.
Seamlessly storing your samples safely plates/well plates as well. After the thermal cycling, scientists cap the well plate with a special film - designed to keep those fresh samples safe from other labware. Sealing well plates ensures that the samples will remain safe while a scientist is not present to examine them.
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